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New year resolution

Losing weight, self improvement and having quality time with family and friends has always become the top resolution for most people when  new year is around the corner. But do you know how many people achieved this? According to one study from University of Scranton, a private uni in Pennsylvania, only 8% achieved their resolution.

Why do you think that so many people failed? What is the the secret of those who are succeed?

That is because we probably not good at goal setting. The key is to keep the list simple,short and more relevant, and of course write it down more specifically!

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If you want 2017 to be your year….

  •  Don’t just sit on the couch and wait for it-
  • GO OUT- Make a change-
  • Smile more
  • Be excited
  • DO NEW THINGS
  • Throw away what you have been cluttering
  • UNFOLLOW NEGATIVE PEOPLE on social media and live a life!
  • Go to BED EARLY WAKE UP early
  • Be FIERCE
  • Don’t gossip
  • Show more gratitude
  • Do things that challenge you
  • …..and Be BRAVE!

A reminder for me too..=) Have a great day ahead!

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The beauty of carbon

Hello everyone. I found this beautiful short story from my random search on carbon..

 

The sheikh asked me “What is the cheapest rock on this planet?”; I replied “Coal”

“And what is the most expensive?” I replied Diamonds.

The sheikh answered “And what are both made of?” I answered “Carbon.”

He continued, “That’s right and do you know what the only difference between those two? Pressure.

Pressure created beautiful things and when Allah swt tests you with difficult situations it is because he wants to shape you into the most beautiful things possible.

 

May you have great days ahead and Happy new year!

 

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Trip to Tissue Culture Lab, IoS, Uitm Shah Alam (Part 1)

In the last October 2014, I made a short visit to Tissue Culture Lab in Institute of Science, Uitm Shah Alam. The purpose of the visit is to get myself introduced with the lab facilities before started my PhD-related work there. I also got to meet  the person that I am collaborating with,  Puan Mazatulikhma Mat Zain. She is the scientist that helped me with the project where they practically use neuroblastoma cell lines. Puan Mai is a very humble, nice and helpful person and very easy to work with.

Unfortunately, I cannot share my touring pictures with you because they were all crashed, I will post it later in my next entry in Part 2 ;). So below I will explain more about the cells that I am working with, which is neuroblastoma SH-SY5Y cell lines. According to literature, the cells were derived from embryonic central nervous system (CNS) tissue. When culturing the cells, they can be both adhering and floating cells, but mostly in studies  the adherent cells are utilised while the floating ones were discarded. The use of cells was quite limited because once the cells reached its maturity, they can no longer be propogated. The uniqueness about this cell is that it can be transformed into neuronal-like lines when induced with retinoic acid (RA) or Vitamin A derivative. Having these two different cell phenotypes will help us broaden our research whereby comparison of these cells can be made.

 

Picture 1

The picture above showed the undifferentiated version of SH-SY5Y cell, where the phenotype corresponds as the “N” type (neuroblast-like cells). The cells have tendency to grow in clusters and may form rounded cells on to of another (showed with arrow).

 

Picture 2

Upon addition of RA, the cells begin to show “S” type shape (epithelial-like cells), and they do not form cluster as the undifferentiated version. The shape is more pyramidal and neurites begin to extend. Retinoic acid is known to possess the most powerful cellular differentiation-promoting properties.

More explanation will be included later on how we can utilized these cells of cancer research activities.

 

References:

http://www.atcc.org/products/all/CRL-2266.aspx#generalinformation

J. Kovalevich and D. Langford. 2013. Consideration for the use of SH-SY5Y neuroblastoma cells in neurobiology. Neuronal Cell Culture. Methods in Molecular Biology Volume 1078, 2013, pp 9-211078:9-21. 

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